Fig 1: Hic1/HIC1 is expressed in the syndetome and sclerotome of developing somites.a Schematic of Hic1nLacZ allele. b Representative whole-mount X-gal staining of Hic1+/nLacZ embryos at the indicated time points. Forelimb buds outlined in white. Arrowheads: Black- hypaxial sclerotomes, white- epaxial sclerotomes, red- Hic1 expression within the central region of forelimb bud. Scale bars, 1 mm. c Immunofluorescence staining with a-HIC1 and a-PAX3 on E10.5 embryos at the forelimb level. Dotted box highlights area inset in adjoining panels. n = 3 embryos from independent litters. Scale bars, 100 µm, 20 µm inset. d Immunofluorescence staining with a-PAX7 on E10.5 Scx-GFP embryos. n = 3 embryos from independent litters. Scale bar, 20 µm. e Immunofluorescence staining with a-HIC1 and a-PAX1 on E10.5 embryos. Dotted box highlights area inset in adjoining panel. n = 3 embryos from independent litters. Scale bars, 100 µm, 20 µm inset. D, dorsal; L, lateral; M, medial; nt, neural tube; V, ventral. f Schematic representation of Hic1+ MP migration from the somite.
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